There are more than 200 species of plants and animals at Harbury including several that are threatened with extinction in Victoria.
Threatened plants at Harbury include Powelltown Correa (Correa reflexa var. lobata), Long Pink-bells (Tetratheca stenocarpa), Silky Fan-fern (Sticherus tener), Oval Fork-fern (Tmesipteris ovata) and Slender Fork-fern (Tmesipteris elongata).
Threatened animals known to live and breed at Harbury include the Greater Glider (Petauroides volans) and Powerful Owl (Ninox strenua). Sooty Owl (Tyto tenebricosa) has been observed at the site and is present throughout the greater landscape.